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<jats:title>ABSTRACT</jats:title><jats:p><jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Chlamydia trachomatis</jats:named-content>causes chronic inflammatory diseases of the eye and genital tract and has global medical importance. The chlamydial plasmid plays an important role in the pathophysiology of these diseases, as plasmid-deficient organisms are highly attenuated. The cryptic plasmid carries noncoding RNAs and eight conserved open reading frames (ORFs). To understand plasmid gene function, we generated plasmid shuttle vectors with deletions in each of the eight ORFs. The individual deletion mutants were used to transform chlamydiae and the transformants were characterized phenotypically and at the transcriptional level. We show that<jats:italic>pgp1</jats:italic>, -<jats:italic>2</jats:italic>, -<jats:italic>6</jats:italic>, and -<jats:italic>8</jats:italic>are essential for plasmid maintenance, while the other ORFs can be deleted and the plasmid stably maintained. We further show that a<jats:italic>pgp4</jats:italic>knockout mutant exhibits an<jats:italic>in vitro</jats:italic>phenotype similar to its isogenic plasmidless strain, in terms of abnormal inclusion morphology and lack of glycogen accumulation. Microarray and qRT-PCR analysis revealed that Pgp4 is a transcriptional regulator of plasmid-encoded<jats:italic>pgp3</jats:italic>and multiple chromosomal genes, including the glycogen synthase gene<jats:italic>glgA</jats:italic>, that are likely important in chlamydial virulence. Our findings have major implications for understanding the plasmid's role in chlamydial pathogenesis at the molecular level.</jats:p>

Original publication

DOI

10.1128/iai.01305-12

Type

Journal article

Journal

Infection and Immunity

Publisher

American Society for Microbiology

Publication Date

03/2013

Volume

81

Pages

636 - 644