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The ECL gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. It is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ECL) detection reaction; the light output is captured on blue-light sensitive film. The application of the system to a range of standard molecular biology hybridization techniques is described.

Type

Journal article

Journal

BioTechniques

Publication Date

05/1990

Volume

8

Pages

564 - 570

Addresses

Amersham International plc., Amersham, Bucks, U.K.

Keywords

Multiple Myeloma, Horseradish Peroxidase, Nucleic Acids, Nucleic Acid Probes, Blotting, Northern, Blotting, Southern, Luminescent Measurements, Nucleic Acid Hybridization, Genes, ras