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Microscopy

As part of the Microscopy Facilities made available by the Core, the laboratory houses the following fluorescence microscopes and related imaging facilities for communal use within the Centre:

  • A Zeiss 510 Meta confocal microscope. Available for all staff and students
  • A wide-field inverted Nikon TE2000U fluorescence microscope. Available for all staff and students
  • An Olympus BX-51 upright wide-field microscope for imaging samples on glass slides. This microscope is primarily for the use of the Chromosome Dynamics and Cellular-Imaging Core staff, as part of their Core service provision.
  • A Leica TCS SP8 confocal with optional FLIM+FCS hybrid detectors and white light laser. Available for all staff and students.
  • A Zeiss Cell Observer Spinning Disc Confocal microscope. It is available for all staff and students, although they will have undertake a 2 day safety course to allow them access to the OPIC cat 3 suite.
  • A Zeiss Elyra TIRF Confocal fluorescence microscope. Available for all staff and students.
  • Access to the Core's Leica CM-3050-S Cryostat. Available for all staff and students
  • Stand-alone image analysis and processing PC workstations. Available for all staff and students.

Services provided

  • Day to day care of the instruments
  • General technical support and help with troubleshooting
  • Training sessions on the microscope(s) of choice
  • Advice on microscopy, live cell imaging, image acquisition, image processing, image analysis and photo editing
  • Advice on advanced microscope techniques such as FLIM, FRAP and FRET
  • Advice on the design and practical execution of immunofluorescence experiments (including provision of protocols, sample confocal dishes, and few aliquots of key reagents to start off)