Cellular Imaging Core Services and Cost Recovery Charges
As part of the Microscopy Facilities made available by the Core, the laboratory houses the following fluorescence microscopes and related imaging facilities for communal use within the Centre:
- A Zeiss 510 Meta confocal microscope. Available for all staff and students
- A wide-field inverted Nikon TE2000U fluorescence microscope. Available for all staff and students
- An Olympus BX-51 upright wide-field microscope for imaging samples on glass slides. This microscope is primarily for the use of the Chromosome Dynamics and Cellular-Imaging Core staff, as part of their Core service provision.
- A Leica TCS SP8 confocal with optional FLIM+FCS hybrid detectors and white light laser. Available for all staff and students.
- A Zeiss Cell Observer Spinning Disc Confocal microscope. It is available for all staff and students, although they will have undertake a 2 day safety course to allow them access to the OPIC cat 3 suite.
- A Zeiss Elyra TIRF Confocal fluorescence microscope. Available for all staff and students.
- Access to the Core's Leica CM-3050-S Cryostat. Available for all staff and students
- Stand-alone image analysis and processing PC workstations. Available for all staff and students.
- Day to day care of the instruments
- General technical support and help with troubleshooting
- Training sessions on the microscope(s) of choice
- Advice on microscopy, live cell imaging, image acquisition, image processing, image analysis and photo editing
- Advice on advanced microscope techniques such as FLIM, FRAP and FRET
- Advice on the design and practical execution of immunofluorescence experiments (including provision of protocols, sample confocal dishes, and few aliquots of key reagents to start off)