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The new Zeiss Cell Observer Spinning Disc Confocal is located in the biological Catagory 3 classified suite at the Oxford Particle Imaging Centre, STRUBI, in the WTCHG building.  

zeiss-spinning-disk.jpg

General Specifications

  • Zeiss Observer Z1 Inverted Microscope
  • NewPort Vision IsoStation anti-vibration isolation table
  • Yokogawa CSU-X1 Filter Wheel and Spinning Disk Unit
  • Photometrics Evolve 512 Delta EM-CCD camera (512 x 512 pixels)
  • Applied Scientific MS2000 Piezo fast motorised Z focus (150μm range)
  • Applied Scientific motorised XY stage
  • Stage insert for glass slides and 3.5cm Petri dishes
  • Definite Focus with NIR LED Hardware Focus to eliminate thermal drift
  • PeCon Temp Module S1 and XL Multi S1 live cell stage incubator (rated 3oC to 45oC)
  • 10x EC Plan Neofluar NA 0.3 air objective  
  • 40x C-Apochromat NA 1.2 water-immersion objective (with coverslip correction collar)
  • 63x C-Apochromat NA 1.2 UV-VIS-IR water-immersion objective (with coverslip correction collar)
  • 100x Plan Apochromat NA 1.4 oil-immersion objective
  • Zeiss HXP-120V short-arc fluorescence Hg lamp and controller (2,000h)
  • DAPI, GFP and DSRed diochroic filters for eyepiece viewing
  • 405nm line DAPI laser
  • 480nm line GFP and 468nm line DsRed laser
  • Zeiss ZEN Blue image aquisition software

To view the Ziess spinning disk *.czi image files on your office PC you will need to download and install Zeiss's Zen Lite program (Windows PCs only). Download the Core's Abridged Instructions for getting started and Zeiss Cell Observer Brochure here. Further help is available as pdf's on the Spinning Disk's PC System C: drive and via in-program help with the Zeiss Zen Blue software.

Access to the microscope is via Robin Parsons in OPIC (biological classification level 3 containment health and safety) and the WTCHG Cellular Imaging Microscopy Core (for microscope training and booking).