Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.
Skip to main content

 

Protein Production UK (PPUK) is a new initiative in the Research Complex at Harwell that addresses sample preparation for challenging proteins and multi-component complexes. PPUK will be part of the Rosalind Franklin Institute and closely linked to the Central facilities on the Harwell campus. PPUK aims to develop new and innovative methods and to work in partnership with academic groups and industry to enable uptake of the technology.

PPUK has an established pipeline for protein production based on the technology developed by the Oxford Protein Production Facility (OPPF), involving construction of multi-purpose vectors (e.g. for producing complexes, and membrane proteins), high-throughput expression screening in different cell hosts (microbial, insect and mammalian cells) combined with scale-up to multi-litre culture volumes and downstream processing. New technology platforms that are under development include production of single-domain antibodies for the stabilisation of membrane proteins for analysis by cryo-EM.

To see the protein production line in action and how we crystallize proteins, please watch the video belowk: http://www.youtube.com/watch?v=joWNZDBqrlQ

Our team

Selected publications