Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Original publication

DOI

10.1091/mbc.01-12-0569

Type

Journal article

Journal

Molecular biology of the cell

Publication Date

06/2002

Volume

13

Pages

2069 - 2079

Addresses

Istituto di Genetica Molecolare del Consiglio Nazionale delle Ricerche, 27100 Pavia, Italy. biamonti@gbe.pv.cnr.it

Keywords

Cell Line, COS Cells, Hela Cells, 3T3 Cells, Chromosomes, Human, Pair 9, Chromosomes, Human, Pair 12, Chromosomes, Human, Pair 15, Cell Nucleus, Heterochromatin, Animals, Cercopithecus aethiops, Humans, Mice, Microscopy, Electron, Fluorescent Antibody Technique, Indirect, Transfection, Mitosis, Stress, Mechanical, Cricetinae