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We have cloned a Chinese hamster chromosome-specific repeated sequence (SatCH5). This satellite is composed of a 33-bp unit organized in two extended tandem arrays. It is localized at the centromere and at the short-arm subtelomere of chromosome 5. Altogether, SatCH5 covers about 1-2 Mb per diploid genome and is not present in other species, including the Syrian hamster and mouse. Since it is known in the Chinese hamster and numerous other vertebrate species that telomeric (TTAGGG)n repeats are localized at the centromeres of several chromosomes, we studied the localization of SatCH5 relative to (TTAGGG)n sequences. Using two-color fluorescence in situ hybridization on stretched chromosomes and on DNA fibers, we have shown that at the centromere of chromosome 5 SatCH5 and the (TTAGGG)n arrays are contiguous. SatCH5 is the first chromosome-specific repetitive sequence located at both the pericentromeric and subtelomeric regions of the same chromosome.

Original publication

DOI

10.1159/000015171

Type

Journal article

Journal

Cytogenetics and cell genetics

Publication Date

01/1998

Volume

83

Pages

281 - 286

Addresses

Dipartimento di Genetica e Microbiologia "A. Buzzati-Traverso," Università degli Studi di Pavia, Pavia, Italy.

Keywords

CHO Cells, Chromosomes, Centromere, Telomere, Animals, DNA, DNA, Satellite, In Situ Hybridization, Fluorescence, Sequence Analysis, DNA, Base Sequence, Repetitive Sequences, Nucleic Acid, Sequence Homology, Nucleic Acid, Molecular Sequence Data, Cricetinae