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Rapid-sedimenting DNA-membrane complexes were obtained from both Bacillus subtilis and Escherichia coli by a method involving gentle lysis followed by restriction enzyme digestion and sucrose gradient fractionation. These complexes were substantially enriched in chromosomal origin DNA, and in B. subtilis, the complexes were enriched in penicillin-binding proteins relative to that of the total membrane. Such complexes may represent procaryotic membrane domains which are topographically and functionally distinct.


Journal article


Journal of bacteriology

Publication Date





192 - 200


Cell Membrane, Chromosomes, Bacterial, Bacillus subtilis, Escherichia coli, Carboxypeptidases, Muramoylpentapeptide Carboxypeptidase, Penicillin-Binding Proteins, Peptidyl Transferases, Hexosyltransferases, Bacterial Proteins, Carrier Proteins, DNA, Bacterial, Molecular Weight