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<ns4:p>The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of <ns4:italic>Escherichia coli</ns4:italic> K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of <ns4:italic>E. coli</ns4:italic> from MARC are already underway to identify ways to improve and enhance MinION performance.</ns4:p>

Original publication

DOI

10.12688/f1000research.7201.1

Type

Journal article

Journal

F1000Research

Publisher

F1000 ( Faculty of 1000 Ltd)

Publication Date

15/10/2015

Volume

4

Pages

1075 - 1075