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We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis -acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10–1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis -regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.

Original publication

DOI

10.1098/rstb.2012.0361

Type

Journal article

Journal

Philosophical Transactions of the Royal Society B: Biological Sciences

Publisher

The Royal Society

Publication Date

19/06/2013

Volume

368

Pages

20120361 - 20120361