Human leukocyte antigen alleles associate with COVID-19 vaccine immunogenicity and risk of breakthrough infection
Mentzer AJ., O’Connor D., Bibi S., Chelysheva I., Clutterbuck EA., Demissie T., Dinesh T., Edwards NJ., Felle S., Feng S., Flaxman AL., Karp-Tatham E., Li G., Liu X., Marchevsky N., Godfrey L., Makinson R., Bull MB., Fowler J., Alamad B., Malinauskas T., Chong AY., Sanders K., Shaw RH., Voysey M., Cavey A., Minassian A., Stuart A., Khozoee B., Hanumunthadu B., Angus B., Smith CC., Turnbull I., Kwok J., Emary KRW., Cifuentes L., Ramasamy MN., Cicconi P., Finn A., McGregor AC., Collins AM., Smith A., Goodman AL., Green CA., Duncan CJA., Williams CJA., Ferreira DM., Turner DPJ., Thomson EC., Hill H., Pollock K., Toshner M., Lillie PJ., Heath P., Lazarus R., Sutherland RK., Payne RO., Faust SN., Darton T., Libri V., Anslow R., Provtsgaard-Morys S., Hart T., Beveridge A., Adlou S., Snape MD., Pollard AJ., Lambe T., Knight JC.
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine immunogenicity varies between individuals, and immune responses correlate with vaccine efficacy. Using data from 1,076 participants enrolled in ChAdOx1 nCov-19 vaccine efficacy trials in the United Kingdom, we found that inter-individual variation in normalized antibody responses against SARS-CoV-2 spike and its receptor-binding domain (RBD) at 28 days after first vaccination shows genome-wide significant association with major histocompatibility complex (MHC) class II alleles. The most statistically significant association with higher levels of anti-RBD antibody was HLA-DQB1*06 (P = 3.2 × 10−9), which we replicated in 1,677 additional vaccinees. Individuals carrying HLA-DQB1*06 alleles were less likely to experience PCR-confirmed breakthrough infection during the ancestral SARS-CoV-2 virus and subsequent Alpha variant waves compared to non-carriers (hazard ratio = 0.63, 0.42–0.93, P = 0.02). We identified a distinct spike-derived peptide that is predicted to bind differentially to HLA-DQB1*06 compared to other similar alleles, and we found evidence of increased spike-specific memory B cell responses in HLA-DQB1*06 carriers at 84 days after first vaccination. Our results demonstrate association of HLA type with Coronavirus Disease 2019 (COVID-19) vaccine antibody response and risk of breakthrough infection, with implications for future vaccine design and implementation.