The evolutionary dynamics of Oropouche Virus in South America
Gutierrez B., Wise EL., Pullan ST., Logue CH., Bowden TA., Escalera-Zamudio M., Trueba G., Nunes MRT., Faria NR., Pybus OG.
<jats:p>The Amazon basin is home to numerous arthropod-borne viral pathogens that cause febrile disease in humans. Among these, <jats:italic>Oropouche orthobunyavirus</jats:italic> (OROV) is a relatively understudied member of the genus <jats:italic>Orthobunyavirus</jats:italic>, family <jats:italic>Peribunyaviridae</jats:italic>, that causes periodic outbreaks in human populations in Brazil and other South American countries. Although several studies have described the genetic diversity of the virus, the evolutionary processes that shape the OROV genome remain poorly understood. Here we present a comprehensive study of the genomic dynamics of OROV that encompasses phylogenetic analysis, evolutionary rate estimates, inference of natural selective pressures, recombination and reassortment, and structural analysis of OROV variants. Our study includes all available published sequences, as well as a set of new OROV genomes sequences obtained from patients in Ecuador, representing the first set of genomes from this country. Our results show differing evolutionary processes on the three segments that comprise the viral genome. We infer differing times of the most recent common ancestors (TMRCAs) of the genome segments and propose this can be explained by cryptic reassortment. We also present the discovery of previously unobserved putative N-linked glycosylation sites, and codons that evolve under positive selection on the viral surface proteins, and discuss the potential role of these features in the evolution of OROV through a combined phylogenetic and structural approach.</jats:p> <jats:p><jats:bold>Importance</jats:bold> The emergence and re-emergence of pathogens such as Zika virus (ZIKV), Chikungunya virus (CHIKV) and yellow fever virus (YFV) have drawn attention towards other co-circulating arboviruses in South America. Oropouche virus (OROV) is a poorly-studied pathogen responsible for over a dozen outbreaks since the early 1960s, and represents a public health burden to countries such as Brazil, Panama and Peru. OROV is likely underreported as its symptomatology can be easily confounded with other febrile illnesses (e.g. dengue fever and leptospirosis), and point-of-care testing for the virus is still uncommon. With limited data, there is a need to optimise the information currently available. Analysis of OROV genomes can help us understand how the virus circulates in nature and can reveal the evolutionary forces that shape the genetic diversity of the virus, which has implications for molecular diagnostics and the design of potential vaccines.</jats:p>