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<jats:title>Abstract</jats:title><jats:p>The transcriptomic and proteomic characterisation of CD4<jats:sup>+</jats:sup> T cells at the single-cell level has been performed traditionally by two largely exclusive types of technologies: single cell RNA-sequencing (scRNA-seq) technologies and antibody-based cytometry. Here we demonstrate that the simultaneous targeted quantification of mRNA and protein expression in single-cells provides a high-resolution map of human primary CD4<jats:sup>+</jats:sup> T cells, and identified precise trajectories of Th1, Th17 and regulatory T-cell (Treg) differentiation in blood and tissue. Furthermore, the sensitivity provided by this massively-parallel multi-omics approach revealed novel insight into the mechanism of expression of CD80 and CD86 on the surface of activated CD4<jats:sup>+</jats:sup> Tregs and demonstrate their potential to identify recently activated T cells in circulation. This transcriptomic and proteomic hybrid technology provides a cost-effective solution to dissect the heterogeneity of immune cell populations, including more precise and detailed descriptions of the differentiation and activation of circulating and tissue-resident cells in response to therapies and in stratification of patients.</jats:p>

Original publication

DOI

10.1101/706275

Type

Journal article

Publisher

Cold Spring Harbor Laboratory

Publication Date

18/07/2019