Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Sialic acid acetylesterase (SIAE) removes acetyl moieties from the carbon 9 and 4 hydroxyl groups of sialic acid and recently a debate has been opened on its association to autoimmunity. Trying to get new insights on this intriguing enzyme we have studied siae in zebrafish (Danio rerio). In this teleost siae encodes for a polypeptide with a high degree of sequence identity to human and mouse counterparts. Zebrafish Siae behavior upon transient expression in COS7 cells is comparable to human enzyme concerning pH optimum of enzyme activity, subcellular localization and glycosylation. In addition, and as already observed in case of human SIAE, the glycosylated form of the enzyme from zebrafish is released into the culture media. During embryogenesis, in situ hybridization experiments demonstrate that siae transcript is always detectable during development, with a more specific expression in the central nervous system, in pronephric ducts and liver in the more advanced stages of the embryo development. In adult fish an increasing amount of siae mRNA is detectable in heart, eye, muscle, liver, brain, kidney and ovary. These results provide novel information about Siae and point out zebrafish as animal model to better understand the biological role(s) of this rather puzzling enzyme in vertebrates, regarding immune system function and the development of central nervous system.

Original publication

DOI

10.1093/glycob/cwx068

Type

Journal article

Journal

Glycobiology

Publication Date

10/2017

Volume

27

Pages

938 - 946

Addresses

Department of Molecular and Translational Medicine (DMTM), University of Brescia, Viale Europa 11, 25123 Brescia, Italy.

Keywords

Liver, Kidney, Nervous System, COS Cells, Animals, Zebrafish, Cercopithecus aethiops, Humans, Acetylesterase, Zebrafish Proteins, RNA, Messenger, Gene Expression Regulation, Developmental, Sequence Homology, Nucleic Acid, Genome