Dr, Group Head / PI, Fellow, Member of congregation and Supervisor
Methodological Quantification of the Spatio-Temporal Regulation of cell-factors controlling Endocytic Routes during Virus Entry
The study of entry mechanisms of enveloped viruses and how they are targeted to different endocytic routes, from internalization to trafficking and fusion is crucial for many reasons: first, virus-cell interactions constitutes an area that is not completely explored and novel strategies to develop drugs and vaccines capable of blocking infection are needed, also viruses are interesting means to deliver drugs and macromolecules within the cell and finally, they are important tools to better understand new mechanisms and concepts related to virus entry (i.e. endocytosis) in structural and molecular biology.
We propose a unique combination of approaches based on quantitative fluorescence microscopy among others; to unveil the spatial and temporal regulation of the host-cell factors implied in endocytic viral entry. We will produce a quantitative microscopy-based framework that will allow to systematically studying how different viruses enter the cell via different routes of endocytosis, and also to discover how different endocytic pathways are regulated in the presence/absence of pathogens.
A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Iliopoulou M. et al, (2018), Nat struct mol biol, 25, 814 - 822
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software.
Nolan R. et al, (2018), Journal of visualized experiments : jove
ijtiff: An R package providing TIFF I/O for ImageJ users
Nolan R. and Padilla-Parra S., (2018), Journal of open source software, 3, 633 - 633
Detrending: How to Correct Images for Bleaching
Nolan R. et al, (2018), Biophysical journal, 114, 345A - 345A
Detecting protein aggregation and interactions in live cells: a guide to Number and Brightness
Rory Nolan None. et al, (2017), Methods