Dr Daniela Moralli
De novo human artificial chromosomes (HAC) are small, extrachromosomal elements, which possess a centromere and are able to replicate and segregate correctly as stable chromosomes. De novo HAC are formed by delivering vectors carrying centromere specific DNA (alpha satellite DNA) to target cells. Because they behave as autonomous chromosomes they can be used as a model to study complex chromosomal features such as the centromere. The aim of my project is to characterise the HAC structure and chromatin composition in different cells types, both immortalized cell line and stem cells, by using a combination of FISH (Fluorescent in situ hybridization) on metaphase spreads, and chromatin fibres, immunological techniques and chromatin immunoprecipitaion (ChIP).
A further interest is the identification of cellular factors involved in HAC formation and maintenance.
When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping
Sailer S. et al, (2021), Cell & Bioscience, 11
Altering the Binding Properties of PRDM9 Partially Restores Fertility across the Species Boundary
Davies B. et al, (2021), Molecular Biology and Evolution
Aluminum enters mammalian cells and destabilizes chromosome structure and number
Tenan MR. et al, (2021), International Journal of Molecular Sciences, 22
Endogenous aldehyde accumulation generates genotoxicity and exhaled biomarkers in esophageal adenocarcinoma.
Antonowicz S. et al, (2021), Nat Commun, 12
Replacement of surgical vasectomy through the use of wild-type sterile hybrids
Preece C. et al, (2021), Lab Animal, 50, 49 - 52
Molecular biology, cellular biology, cytogenetics
Human artificial chromosomes, centromere, chromatin, chromosome stability