Dr Daniela Moralli
De novo human artificial chromosomes (HAC) are small, extrachromosomal elements, which possess a centromere and are able to replicate and segregate correctly as stable chromosomes. De novo HAC are formed by delivering vectors carrying centromere specific DNA (alpha satellite DNA) to target cells. Because they behave as autonomous chromosomes they can be used as a model to study complex chromosomal features such as the centromere. The aim of my project is to characterise the HAC structure and chromatin composition in different cells types, both immortalized cell line and stem cells, by using a combination of FISH (Fluorescent in situ hybridization) on metaphase spreads, and chromatin fibres, immunological techniques and chromatin immunoprecipitaion (ChIP).
A further interest is the identification of cellular factors involved in HAC formation and maintenance.
Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer.
Yeow ZY. et al, (2020), Nature, 585, 447 - 452
ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair
Wells D. et al, (2020), eLife, 9
The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates
Hinch AG. et al, (2020), Molecular Cell, 79, 689 - 701.e10
Gene expressing human artificial chromosome vectors: Advantages and challenges for gene therapy.
Moralli D. and Monaco ZL., (2020), Experimental cell research
A living biobank of ovarian cancer ex vivo models reveals profound mitotic heterogeneity.
Nelson L. et al, (2020), Nature communications, 11
Molecular biology, cellular biology, cytogenetics
Human artificial chromosomes, centromere, chromatin, chromosome stability