Dr Daniela Moralli
De novo human artificial chromosomes (HAC) are small, extrachromosomal elements, which possess a centromere and are able to replicate and segregate correctly as stable chromosomes. De novo HAC are formed by delivering vectors carrying centromere specific DNA (alpha satellite DNA) to target cells. Because they behave as autonomous chromosomes they can be used as a model to study complex chromosomal features such as the centromere. The aim of my project is to characterise the HAC structure and chromatin composition in different cells types, both immortalized cell line and stem cells, by using a combination of FISH (Fluorescent in situ hybridization) on metaphase spreads, and chromatin fibres, immunological techniques and chromatin immunoprecipitaion (ChIP).
A further interest is the identification of cellular factors involved in HAC formation and maintenance.
Replacement of surgical vasectomy through the use of wild-type sterile hybrids
Preece C. et al, (2021), Lab Animal
Genomic Instability Is an Early Event in Aluminium-Induced Tumorigenesis.
Mandriota SJ. et al, (2020), International journal of molecular sciences, 21
Specific Mechanisms of Chromosomal Instability Indicate Therapeutic Sensitivities in High-Grade Serous Ovarian Carcinoma.
Tamura N. et al, (2020), Cancer research, 80, 4946 - 4959
Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer.
Yeow ZY. et al, (2020), Nature, 585, 447 - 452
ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair
Wells D. et al, (2020), eLife, 9
Molecular biology, cellular biology, cytogenetics
Human artificial chromosomes, centromere, chromatin, chromosome stability