|Fluorescent 1 and 2 cell embryos|
|A single ES cell colony expressing Green Fluorescent Protein growing on a fibroblast feeder layer|
|Microinjection of embryonic stem cells into a blastocyst|
Dr, Transgenic Core Head
Genetically modified models represent one of the most powerful methods of functional gene analysis in vivo. Furthermore, the ability to introduce specific mutations into the genome enables models of human disease to be generated, facilitating insights into the pathophysiology of disease and providing a model with which therapeutic strategies and diagnostic tools can be optimized.
Our group provides groups within Oxford University access to transgenic technologies both on a fee-for-service type arrangement and on a collaborative basis. Technologies offered include embryo microinjection, embryonic stem cell transfection, Knock-out/-in construct design and in vivo shRNA mediated gene Knock-down. In addition, embryo rederivation and cryoconservation services are offered to facilitate the management, transfer and security of genetically modified strains.
The research activity of the group is focused on the development of novel methodologies for the generation of genetically modified models. The aims being to improve the reliability of the technology and to reduce the animal cost of research involving genetically modified models.
Factors influencing meiotic recombination revealed by whole-genome sequencing of single sperm
Hinch AG. et al, (2019), Science, 363, eaau8861 - eaau8861
An essential role for the Zn2+ transporter ZIP7 in B cell development
Anzilotti C. et al, (2019), Nature Immunology, 20, 350 - 361
Single-copy expression of an amyotrophic lateral sclerosis-linked TDP-43 mutation (M337V) in BAC transgenic mice leads to altered stress granule dynamics and progressive motor dysfunction
Gordon D. et al, (2019), Neurobiology of Disease, 121, 148 - 162
Loss of ZnT8 function protects against diabetes by enhanced insulin secretion
Dwivedi OP. et al, (2018)
Mechanics of mouse blastocyst hatching revealed by a hydrogel-based microdeformation assay
Leonavicius K. et al, (2018), Proceedings of the National Academy of Sciences, 115, 10375 - 10380