Search results for "Transcriptome (RNA)"

Directional RNA Libraries: Orientation and Strandedness

24/09/2020

Strandedness and Orientation

Once data has been received and analysed, it is often time to upload it to a database, many of which ask for metadata to be added along with the raw data. OGC offers…more

Primary Data QC

Ensuring high data quality through a rigorous QC at the OGC

The quality of the sequencing data generated at the Oxford Genomics Centre (OGC) is analysed using an in-house developed primary Quality Control (QC) pipeline. Depending on the library type,…more

Read-to-index mis-assignment

24/09/2020

The Oxford Genomics Centre (OGC) has been generating sequencing data using Illumina’s high-throughput technologies since 2008. Over these years the combined experience within the group and relationship with its end users has enabled the OGC…more

Approaches to RNA sequencing

24/09/2020

We prepare and sequence a high volume of libraries specifically tailored for transcriptional data analysis. We’ve tested and compared the leading kits on the market, and with a mind for consistency we have successfully automated much of the workflows.…more

Quality Checking Metric Examples

21/04/2017

Submitting samples

When we receive samples, whether they are DNA, RNA or Premade libraries, they first undergo our quality control (QC) assays before being processed by the Library Preparation Team or Sequencing Team. Performing these assays…more

A colorimetric method to determine efficiency of FACS sorting

28/03/2017

The colorimetric method (Rodrigues et al, 2016) can be used to determine the efficiency of FACS sorting into 96- and 384-well plates. It’s an inexpensive method that uses 3, 3′, 5, 5′- Tetramethylbenzidine (TMB) and…more