Routine in vivo adult zebrafish scan procedures

Note:

Anatomical 3D imaging 

Scan time: ~ 30 min + 10 min prep time

Example: Assessment of ventricular tissue changes at the lesion during heart regeneration

Note that this method could be adapted to other aquatic species, and other organs.

zebrafish_orthogonal
Above: 3D imaging of a live 1 year old zebrafish at 31μm isotropic resolution. The images show a zebrafish in three orthogonal planes from mid-eye to mid swim-bladder level, with the heart outlined in green.