Ben_Fairfax_Circos

RNA-Seq Analysis

EIF2AK1_alt_splicing_RNA_SeqFigure shows alternative splicing occuring in exons 2 and 3  of EIF2AK1 between normoxic and hypoxic conditions.

RNA-sequencing:

RNA-seq is a deep sequencing process that enables the  profiling of the entire transcriptome in any organism. This type of sequencing is commonly used in projects that aim to either quantify the levels of gene expression, detect differential expression or detect alternative splicing in a sample.

The Oxford Genomics Centre's  bioinformatics unit offer several types of analyses to assist the customer in the interpretation of RNA-seq data. The following files will be provided with RNA-seq results:

 

  • Primary QC report  - A file of general QC metrics for each sample sequenced.
  • Secondary QC report  - A file of QC metrics to assess the quality and specificity of the reads mapping to the transcriptome in each sample.
  • FASTQ file - A file of unmapped sequenced reads for each sample.
  • A BAM file - An alignment report of sequenced read mapping results.
  • Counts file  - A file containing the number of reads mapping to annotated genes.

 

Optional analyses available:

  • Differential expression
  • Alternative splicing
  • GO/pathway enrichment
  • Custom RNA-seq analyses

 

  Contact us

 

Small RNA Profiling & Discovery:

 

Micro-RNA (miRNA) discovery represents a growing field within the biological research community. These short nucleotide sequences are though to play a critical role in gene regulation.

The Oxford Genomics Centre's  bioinformatics unit offer several types of analyses to assist in the discovery of miRNA-based gene regulatory changes within a sample. The following files will be provided with Small RNA-seq data:

 

  • Primary QC report  - A file of general QC metrics for each sample sequenced.
  • Secondary QC report  - A file of QC metrics to assess the quality and specificity of the reads mapping to the genome in each sample.
  • FASTQ file - A file of unmapped sequenced reads for each sample.
  • A BAM file - An alignment report of sequenced read mapping results.
  • Counts file  - A file containing the number of reads mapping to a library of known small RNAs.

 

Optional analyses available: 

  • Analysis of differentially expressed small RNAs.
  • Target gene identification.
  • Custom analyses.

 

  Contact us