The Oxford Genomics Centre as part of the Wellcome Trust Centre for Human Genetics is a partner in the Oxford Consortium for Single-Cell Biology (Oxford ConSiCe Biology), receiving a share in a £4.9M award from the MRC’s Clinical Research Capabilities and Technologies Initiative which will be used to develop single-cell capabilities (see news item: Developing Single-Cell Capability).
We offer a wide and growing range of genomics applications for single cells, including RNA-seq, gene expression profiling by qPCR and DNA amplification for whole-genome or targeted (exome or PCR-based analysis). Applications are supported by the Fluidigm C1 Single-Cell Auto Prep and Polaris integrated research systems, or in conventional multi-well plates using FACS (cell sorting) combined with low-volume, automated liquid handling.
The 10X Genomics Chromium™ Controller is a platform designed to deliver highly parallel sample partitioning and molecular barcoding. It supports a variety of applications, including the well-established 10X Genomics Linked-Read technology for genome analysis and the existing Chromium™ Single Cell 3’ Solution for single cell transcriptomics, allowing cell characterization and gene expression profiling of hundreds to millions of cells.
The C1 cell preparation system can isolate up to 96 cells and then process them to produce pre-amplified cDNA or amplified genomic DNA for downstream analysis, including sequencing on the Illumina platforms. The most commonly-used application is currently RNA-seq. The whole process from cell capture to sequence-ready libraries can now be automated to quickly and efficiently deliver gene expression data from many diverse cell populations.
This new, simplified workflow uses Fluidigm’s C1 Single-Cell Auto Prep System, Clontech’s SMARTer Ultra Low RNA input Kit for cDNA synthesis and Illumina’s Nextera XT Sample Preparation Kit for library preparation.
We have automated the downstream processing of C1 chips, from cDNA QC to the consolidation of samples from multiple chips to standard 96-well format plates, followed by library construction, pooling and QC. Cells can be selected for further analysis including sequencing, based on imaging data.
The new integrated Polaris system can be used to select, isolate, dose, culture and process individual cells ready for downstream molecular preparation and analysis. By coordinating cell selection, control of the cell’s biological conditions and immediate analysis of the cell’s genomic response, this system offers the ability to accurately correlate genotype and phenotype.
We also support read-out using the Fluidigm BioMark HD real-time PCR instrument and assay types that include Fluidigm and TaqMan custom assays in addition to RNA-seq.
We offer a range of service entry-points, from submitted cell samples to harvested C1 products and pre-made libraries. We also offer equivalent protocols undertaken on isolated cells, lysed cells and amplified materials in micro-well plates.
If you are interested in this technology please contact us for more details.
SH800Z cell sorter features a novel microfluidics sorting chip integrated with comprehensive fluidics controls to simplify system and sort setup.
A flexible optical design offers up to 4 collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and 6 fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.
OGC offers a complete workflow from cell suspension to sequencing-ready library (SmartSeq2 combined with NexteraXT)
Please contact us if you’re interested in using our cell sorting facility.