Routine In Vivo Mouse Protocols

 

Note:

 

Anatomical imaging (i.e. organs, aorta, pulmonary circulation etc)

Scan time: ~15 mins

Example: Molecular imaging

McAteer, Martina A., et al. "Magnetic resonance imaging of endothelial adhesion molecules in mouse atherosclerosis using dual-targeted microparticles of iron oxide." Arteriosclerosis, thrombosis, and vascular biology 28.1 (2008): 77-83.

Akhtar, Asim M., et al. "In vivo quantification of VCAM-1 expression in renal ischemia reperfusion injury using non-invasive magnetic resonance molecular imaging." PloS one 5.9 (2010): e12800.

 

Global cardiac function (cine-MRI) in juvenile and adult mice

Scan time: ~10 mins

Neonates scan time: ~30mins

Examples: LV mass, LV/ RV function (volumes, EF, SV, CO), infarct size

mouse cine 2

Above: An example frame of a cine experiment in a mouse.

Schneider, Jürgen E., et al. "How to perform an accurate assessment of cardiac function in mice using high-resolution magnetic resonance imaging." Journal of Cardiovascular Magnetic Resonance 8.5 (2006): 693-701.

 

Late Gadolinium Enhancement (LGE)

Scan time: ~15 mins

Example: Early post-infarct scar assessment

late gad enhanced

Bohl, Steffen, et al. "Advanced methods for quantification of infarct size in mice using three-dimensional high-field late gadolinium enhancement MRI." American Journal of Physiology-Heart and Circulatory Physiology 296.4 (2009): H1200-H1208.

 

T1-mapping

Scan time: ~3 mins per slice

Example: Post-ischaemia Area-at-risk (AAR) (requires full validation)

Bohl, Steffen, et al. "Advanced methods for quantification of infarct size in mice using three-dimensional high-field late gadolinium enhancement MRI." American Journal of Physiology-Heart and Circulatory Physiology 296.4 (2009): H1200-H1208.

 

Non-contrast based perfusion measurement

Scan time: ~6 mins per slice

Example: Myocardial blood flow

Campbell‐Washburn, Adrienne E., et al. "Cardiac arterial spin labeling using segmented ECG‐gated Look‐Locker FAIR: Variability and repeatability in preclinical studies." Magnetic resonance in medicine 69.1 (2013): 238-247.

 

1H-MRS (single voxel)

Scan time: < 20 mins

Examples: Creatine, lipid assessment / quantification

Schneider, Jürgen E., et al. "In vivo cardiac 1H‐MRS in the mouse." Magnetic resonance in medicine 52.5 (2004): 1029-1035.

 

23Na-MRSI

Scan time: ~30 mins

Example: Ion homeostasis

23na_figure

Above: An example 3-fold accelerated compressed sensing chemical shift image in a wild-type C57BL/6 mouse.  The 23Na spectroscopic image is shown in colour, overlayed onto a corresponding 1H scout image.  Example phased 23Na spectra corresponding to voxels in the septal myocardium (+) and left ventricular blood (*) are shown on the right; both sectra are plotted on the same scale.  The myocardium is clearly visible in the 23Na image, and the voxel dimensions are approximately 0.47x0.47x2.00 mm.  A concentration reference phantom (CR) has been masked in this image.  The location of the RF coil used to detect the signal is also indicated.

Maguire, Mahon L., et al. "Compressed sensing to accelerate magnetic resonance spectroscopic imaging: evaluation and application to 23 Na-imaging of mouse hearts." Journal of Cardiovascular Magnetic Resonance17.1 (2015): 1.

 

31P-MRSI

Scan time: ~1 hour

Example: High-energy phosphate metabolism (PCr/ATP)

 

31P-MRSI Sat transfer

Scan time: ~2 hours

Example: High energy phosphate metabolism (Creatine Kinase flux)