The main focus of my work within the Mir group is to design and execute experiments with the aim of providing proof-of-principle results for the massively parallel DNA sequencing technology the group is developing. As well as whole human genomic DNA I use a number of models systems (human Beta-globin exons, Cystic Fibrosis Transmembrane Receptor (CFTR) exons, Phi X174 bacteriophage genome) in conjunction with low and high density array technology, including fluorescence single molecule detection and traditional molecular biology techniques (PCR, ligation, gel analysis).
Mir KU, Qi H, Salata O and Scozzafava G. 2009. Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template, Nucleic Acids Res. Jan;37(1):e5. Epub 2008 Nov 16. PMID: 19015154
Pickering J, Bamford A, Godbole V, Briggs J, Scozzafava G, Roe P, Wheeler C, Ghouze F, Cuss S. 2002. Integration of DNA ligation and rolling circle amplification for the homogeneous, end-point detection of single nucleotide polymorphisms. Nucleic Acids Res. Jun 15;30(12):e60. PMID: 12060698
Ghouze F, Scozzafava G, Oreo R, Hughes B, Roe P, Wheeler C, Howe R, Morris S, Comley J. 2002. Flexible and Scalable Automation Solutions For Scoring Single Nucleotide Polymorphisms Using Rolling Circle Amplification, JALA, Jun 1, Volume 7, Issue 3, 70-75.
Rösler A, Bailey L, Jones S, Briggs J, Cuss S, Horsey I, Kenrick M, Kingsmore S, Kent L, Pickering J, Knott T, Shipstone E, Scozzafava G. 2001. Rolling circle amplification for scoring single nucleotide polymorphisms. Nucleosides Nucleotides Nucleic Acids. Apr-Jul;20(4-7):893-4. PMID: 11563139
Durrant I, Benge LC, Sturrock C, Devenish AT, Howe R, Roe S, Moore M, Scozzafava G, Proudfoot LM, Richardson TC et al. 1990. The application of enhanced chemiluminescence to membrane-based nucleic acid detection. Biotechniques. May;8(5):564-70. PMID: 2192738
Research Areas: development of tools for genomics, low cost sequencing technology, laboratory automation.
Keywords: genomics, DNA sequencing, laboratory automation, technology development.
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