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Microscopy Core
The Core
provides fluorescence microscopes for image capture and
quantitative analysis
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A
Zeiss 510 MetaHead
confocal fluorescence microscope in inverted configuration
suitable for glass slides and live cell work.
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A wide-field inverted
Nikon
TE2000U fluorescence microscope fitted with 4x to 60x long
working distance objectives with separate B&W fluorescence
and colour video cameras to image both slides and cells in
culture.
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An
Olympus BX-51 upright
wide-field microscope for imaging samples on glass slides,
suitable for mFISH analysis with Applied Imaging's
Genus software or general image capture/analysis using
MetaMorph acquisition software.
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Plus there are a few
stand-alone image analysis and processing PC workstations
running Applied Imaging Genus, NIS Elements, IPLab,
MetaMorph v7.5
Premiere [offline], ImageJ and
Improvision
Volocity deconvolution software v4.2.
In addition we have an elderly Nikon
CoolPix 995 pixel digital compact camera - with a 2048 x
1536 image resolution and 4x optical zoom .
The Microscopy Core is located on the ground floor, Laboratory 1,
Room's 00/069 and 00/070. Only Microscopy Core trained users
have access to the microscopes in 00/070. The microscopes
can be used by all Wellcome Centre staff, free of charge,
once they have been trained by Core staff. Email us for
further details or for new user microscopy induction at
microscopy@well.ox.ac.uk. Telephone 01865 287568 [internal
ext. 87568] for
the laboratory
00/069. Please read our
Health and Safety document before using the microscopes.
Live Cell Imaging
Upgrade
The Microscopy Core is currently
purchasing an upgrade to the Zeiss LSM510 MetaHead confocal
microscope in order to make it suitable for long-term live cell
or tissue imaging. Please follow
the left button link for more details of the
live cell
incubator system to be installed on the confocal's
inverted Axiovert 200M microscope.
The
Leica CM-3050-S Cryostat
The Microscopy Core has a top of the range
Leica CM-3050-S cryostat for cutting sections from
frozen tissue samples and cell suspensions.
It's particularly suitable for
working with delicate specimens such as brain samples in
neuroscience. The precise specimen orientation and the feed
system via it's step motor produces reproducible thin serial
sections of high quality.
Please click on the buttons below for
more information on our systems
Some links are only
active within the Wellcome Trust Centre intra-net
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