Molecular Cytogenetics
Metaphase
FISH
Metaphase FISH is used to
identify the chromosomal localisation and position of DNA sequences ranging in length from
IMAGE clones to YACs. Chimerism and sequence homology can also be established.
The location of integrated
sequences can be determined via metaphase FISH using probes made from the transfected
products in combination with M-FISH.
Two, three and four colour
FISH is used to determine clone order.
Click here for images
Metaphase FISH
:
Metaphase
with PAC clone : IMAGE clone : Clone ordering : 4colour FISH
Fibre
FISH
FISH to DNA fibres with BAC
and PAC allows rapid visualisation of the extent of overlaps and gaps in contigs.
Small clones can be hybridised against larger YAC clones to confirm clone order over
longer distances.
Fibre FISH with transfected
clones allows the quantitation of integrated sequences and can be used to demonstrate the
final integrity of the construct.
Click here for images
Fibre and
Interphase FISH : 3
colour fibre FISH : BAC clones hybridised with a YAC :
PAC clone Interphase/Fibre
FISH Protocols
For fluorescent in-situ hybridisation [FISH] practical
notes, theory and troubleshooting have a look at at our Core's
FISH protocol web page. Note that most of the links there only
function if you are logged on to our local intra-net.
Our Cost Recovery Charges
The use of our microscopes and image
analysis workstations are free for all Wellcome Centre staff. We operate a
cost recovery system for the molecular cytogenetic services we offer,
although this is a minimal charge and only recovers the cost of the
consumables we have to use for the techniques.
Download our cost
recovery charges as an Adobe
pdf file.
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