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Molecular Cytogenetics
Microscopy
Core
The Post-Genomic FISH Club
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Molecular Cytogenetics and
Microscopy Core
The inter-related aims of
this research facility are to provide investigators at the WTCHG
with (1) Molecular Cytogenetic support and (2) access to
bright-field, fluorescence and confocal microscopy. In addition, the Core serves as a reference source within the
University providing advice and collaboration for research
projects employing fluorescence in situ hybridisation and other
immuno-fluorescence cytological techniques.
Molecular
Cytogenetics
The Core Staff carries out
Fluorescence in situ Hybridization (FISH) experiments and
chromosomal analysis for:
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Verification of chromosomal location of clones/probes
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Identification of chromosomal anomalies and analysis of candidate
regions in patients or individuals who manifest some of
the clinical symptoms of a specific disease
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Characterisation of transgenic loci
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Verification of cell lines authenticity; assessment of their
chromosome content and gene copy number prior to molecular
investigations
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Validation of genome architecture and copy number variation (CNV)
studies.
As well as DNA-FISH, the portfolio
of cytological techniques available in the Core comprises M-FISH, RNA-FISH,
chromosome analysis & karyotyping, immunocytochemistry, immunohistochemistry,
immunoFISH and chromogenic
RNA in situ hybridization on tissue. Follow the
Molecular Cytogenetics link for further details including our cost
recovery charges.
Microscopy
The Core provides microscopy imaging facilities, advice on their use and general technical support.
There is one Zeiss 510 MetaHead confocal and two standard wide-field
fluorescence microscope imaging systems, plus image analysis and process
workstations running Applied Imaging Genus, NIS, IPLab, MetaMorph,
ImagePro Plus [LaserPix], ImageJ and Volocity. For further details
follow the
Microscopy link.
You must be
on the Wellcome Intranet for some of our in-house pdf guide download links to
work.
As well as helping with imaging and analysis at the microscope, upon consultation the Core can
provide with advice on the overall experimental strategy and help with the design and
practical execution of immunostaining experiments.
Staff
Group Head :
Dr.
Emanuela Volpi
Core Staff :
Dr. Keith J Morris [Microscopy Core, Live Cell & Image Processing/Analysis,
Web Pages]
Core Staff :
Natalie Alsamhouri [Cell Culture, FISH, Chromosomal Analysis]
Core Staff :
Dr. Mohammed Yusuf [Cell Culture, FISH, Karyotyping]
For general molecular cytogenetics and microscope
inquiries, and to arrange microscope training, please email us all at
microscopy@well.ox.ac.uk.
Our telephone number's are: 01865 287568 - the Core laboratory, and
01865 287646 - Dr E. Volpi's office. Internal Oxford University staff
need only dial extensions 87568 and 87646 respectively.
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