Blog of a Work Experience Student

By Anna Klucnika

Monday

On my first day, I got briefed on health and safety and shown around the labs. Dianne explained her work researching the genetics behind SLI, which was really interesting as I could put my GCSE biology into action and was able to understand it. I got to extract RNA from cells and measured how much RNA there was by seeing how much light passed through it using a machine. We also collected our saliva samples from which we extracted the DNA. I got to see and use different equipment, like a centrifuge and vortex and learn how to use a precise pipette. It was amazing to actually see a little clump of DNA after we removed it from the cells. I also went to a seminar with Dianne. Although I didn't understand most of it, it was nice to hear terms used in my biology lessons and see another side of what the work includes. I was also given a lab book in which I had to write up what we did in the labs, which everyone does so that if anyone wants to continue your work or if it's needed for patenting.

Tuesday

Using the DNA we extracted the day before, we amplified a short section of DNA using a Polymerase Chain Reaction and then check the length using gel. I got to be really hands-on with everything and Dianne explained exactly what was happening in the PCR. It was really interesting and I got to learn so much more about DNA and genes. I also got to see how the confocal microscope and how they use it to study proteins, which was also fascinating and so much more than I had seen at school.

Wednesday

We looked up what genes the sections of DNA which we amplified were using a genome database and software. I got learn how to use the programme and, again, I learnt loads about genetics. I also got to sit in the team's meeting. Though I didn't understand most of it, it was so interesting to see how the different people worked together and passed on ideas. Even there I learnt so much. I also saw the machines used to sequence DNA and explained how they work, which was amazing.

Thursday

There was another seminar, and, once again, I didn't understand most of it but nevertheless the parts which I did understand were really interesting. We prepared the DNA samples for sequencing and I feel more and more at home in the lab. Completing the lab book is also really nice as it puts everything together and makes a record of what we did. Dianne was also looking at if there was a link between SLI and seasonality of births. It was fascinating to see the data and how it can be used, as well as being able to input my ideas. It was really interesting and made me think about biology in a real life situation.