ATAC-Seq is a relatively new technique that enables the study of chromatin accessibility using a faster approach than DNAse-Seq. It aims to identify DNaseI hypersensitive sites (i.e. all DNA-accessible regions) to determine DNA-binding proteins’ “footprints” and to infer nucleosome positions. ATAC-Seq can be performed either on bulk samples or on single cells.
The following files can be provided with ATAC-Seq projects:
- Primary QC report (general QC metrics on sequencing quality for each sequenced sample)
- FASTQ (raw sequence data) and BAM (alignment) files for each sample
Bespoke downstream ATAC-Seq analyses*:
- Secondary QC report (a set of QC metrics including fragment size distribution, reproducibility of the experiment and genomic features enrichment)
- Full peak annotation (including nearest gene annotation and overlap with Encode’s TFs, DNaseI and other tracks of interest)
- Customised ATAC-Seq downstream analyses
* Please note that bespoke analyses might be subject to availability and resources, please contact us for more information.